5. The Dual-Luciferase ® Reporter Assay or DLR is widely used to study gene transcription and regulation. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. The SpectraMax M5e Reader offers the additional benefit of being certified for Cisbio Bioassays’ HTRF® technology. For more information. Read & analyse. Lysates are then transferred to the assay plate for the detection of total-STING by HTRF. Plate 25 μL of cells in 96 half-well plate in your appropriate medium. Choose from over 50 certified or compatible plate readers More than 50 certified or compatible instruments, and counting! Your lab might even already have one. Make sure to use the set-up for Eu Cryptate. Read & analyse. The Infinite 200 PRO is an easy-to-use multimode plate reader family that offers affordable, high performance detection solutions, referenced in more than 1800 peer reviewed publications. 4. Plate 25 μL of cells in 96 half-well plate in your appropriate medium. The VICTOR ® Nivo ™ system packs all popular detection modes into the industry’s smallest multimode reader footprint. This protocol gives the cells viability and confluence to be monitored. + 4 µL HTRF pre-mixed antibodies. + 4 µL HTRF ® pre-mixed antibo dies 2. For more information about HTRF. 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies. Unlike standard ELISA plate readers, MSD. Remove the plate sealer and read on an HTRF® compatible reader . Depending on cell lines used, it can be necessary to dilute the cell lysate to ensure samples are within the assay linear range FOR HTRF CERTIFIED READERDetection of Total BRD4 with HTRF reagents is performed in a single plate used for plating, stimulation and detection. NC1286225. Lyse cells Read on an HTRF compatible reader . Plus . Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. Specialized filters allow for IR fluorescence detection ranging from 10-40 times more sensitive than conventional monochromator. The SpectraMax ® iD5 Multi-Mode Microplate Reader is HTRF-certified, with an enhanced TRF detection module and filters optimized for HTRF detection. Incubate overnight at RT. 0. For more information. HTRF Signal + 16 µL Standard or Sample 4 µL of pre-mixed TNFα Antibodies ADD READ ANALYSE Incubate 2 hours* at room temperature *Following incubation, the signal remains stable over a period of 24 hours. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. The plate was covered and incubated for one hour at room temperature. Remove the plate seal and read on an HTRF compatible reader. Plate 25 μL of cells in. You can count on our plate readers to bring you the performance, reliability, consistent results, and the best user experience available—across a multitude of applications and a range of detection options. For more information about microplate recommendations, please visit our website • Store the kit at -16°C. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. use of a wide variety of plates or sample matrix formats. Incubate overnight at RT. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. Incubate 4h at RT. Page 30 4. • HTRF®-Certified Reader. FOR HTRF CERTIFIED READER. It comes equipped with our patented LVF Monochromators™, filters, and spectrometer. The EnVision ® multimode plate reader provides exceptional speed, ultra-high throughput, and maximum sensitivity across all detection technologies. These plates can be read on all HTRF certified readers, thus making their implementation in. Even if not listed, some. Incubate 4h at RT. A gradient of signal is apparent prior to plate reader optimization (a) causing a %CV of 9. Plate 25 μL of cells in. It measures at any wavelength, with top and bottom reading for most standard technologies. Cisbio’s 96-well low volume plates can be used with all HTRF assays, for example second messengers, phospho-proteins, cytokines and biomarkers. On BMG LABTECH´s NEPHELOstar Plus, a laser diode (at 635 nm) with a highly collimated beam is used as high-intensity light source. • HTRF plate validated (QC with reference HTRF assays) • Low to high volumes (10 µL to 350 µL) • Can be used with all HTRF compatible readersHTRF combines standard FRET technology with time-resolved measurement of fluorescence, eliminating short-lived background fluorescence. Make sure the setup for Tb Cryptate is used. * For HTRF microplate recommendations, please visit . Evaluation of the performance of the Spark™ multimode reader for far red fluorescence emission. 2 Locking the Plate Carrier Upon closing the software or pushing the plate carrier in/out button for more than three seconds (figure 10), the plate carrier will move into the respective lock position. 96-well culture plateHTRF certified . HTRF combines standard FRET technology with time-resolved measurement of fluorescence, eliminating short-lived background fluorescence. The VICTOR Nivo is a HTRF certified microplate reader. The PHERAstar FSX is the best microplate reader for all HTRF assays: Sensitive, fast and reliable measurements with best Z´ and DeltaF% HTRF-certified with white and black. For more information about HTRF. Remove the plate seal and read on an HTRF compatible reader. CLARIOstar is equipped with a specific optical device which enables the measurement of both 620 nm cryptate and 665 nm acceptor emissions. For HTRF certified reader For more information about HTRF® compatible readers and for set- up recommendations, please visit our website. They can be used in a no-transfer protocol with suspension cells or with a transfer step for adherent cells. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. Based on this customer experience and feedback, the Infinite 200 PRO microplate plate reader has been continuously engineered for excellence. The EnSight<sup>®</sup> multimode plate reader offers HTS Alpha and Ultra-sensitive Luminescence technologies alongside conventional multimode detection technologies. For more information about HTRF ®Detection of Total BRD4 with HTRF reagents is performed in a single plate used for plating, stimulation and detection. Up to 8 decades dynamic range in one measurement with EDR technology. Read & analyse. The perfect microplate reader for everyday biochemical and cell-based assays, it measures at any wavelength, with top and bottom reading of all technologies. Preconfigured HTRF protocols in SoftMax ® Pro Software 7. For more. No washing steps are required. 96-well culture plateThe assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. Enable more assay conditions Define individual reagents to be delivered to each well reducing reagent consumption and allowing for more assay conditions to be explored on a single microplate. culture plate. HTRF Signal + 16 µL 4 µL Standard of pre-mixed IL8 Antibodies or Sample ADD READ ANALYSE Incubate 1 hours* at room temperature *Following incubation, the signal remains stable over a period of 24 hours. 3 and higher include optimized reader settings, as well as data analysis, for faster results. Certified for use with HTRF ®, Alpha, LANCE ® or DELFIA ®. 7ONE-PLATE ASSAY PROTOCOL: Small volume detection plate. The plate is set up for reading and images are acquired according to specified parameters. PerkinElmer offers two microplate readers certified for use with HTRF: Envision. spectramax paradigm system, htrf reagents, spectramax i3 and paradigm readers, HTRF assay, htrf certifiedLysates are then transferred ®to the assay plate for the detection of Total CHK2 by HTRF reagents. The ratio for each well must be calculated individually. PROTOCOL AT A GLANCE. (In the following text, the term YTHDC1 is used frequently as an abbreviation for the m 6 A-recognition domain of YTHDC1). 6% for 1,408 wells and a Z’ value of 0. Results for HTRF Reader Control Kit with black and white microplates. Better sensitivity provides better statistic, less variability among replicates, and a larger assay window. For more information. Not for use in diagnostic procedures. 96-well culture plateFOR HTRF CERTIFIED READER For more information about HTRF. PROTOCOL AT A GLANCE Do not pre-mix the d2 and Cryptate solutions prior to dispensing. SERIAL DILUTIONS . Certified for use with HTRF ®, Alpha, LANCE ® or DELFIA ®. This protocol, HTS designed, allows miniaturization while. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. The new Molecular Devices instrument comes with a standard 1-year depot warranty service. Lysates are ®then transferred to the assay plate for the detection of phospho- Rab10 (Thr73) by HTRF reagents. $352. Cells in each image are identified by SoftMax Pro Software and cell-by-cell HTRF®-certified, DLReady, and SpectraMax® DuoLuc™-certified. For more information about HTRF. For more information about HTRF. FOR HTRF CERTIFIED READER. The HTRF cellular assays were found to be at least 16-fold more sensitive than the Western Blot. Microplate reader assays requiring infrared (IR) or. Fluorescence intensity – Fluorescence intensity is one of the most. Depending on the cell lines used, it may be necessary to dilute the cell lysate to ensure samples are within the assay linear range. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. Conclusion. No washing steps are required. Certified for use with HTRF ®, Alpha, LANCE ® or DELFIA ®. Time-resolved fluorescence was measured switch the SpectraMax i3x and SpectraMax iD5 readers using preconfigured protocols in SoftMax® Pro Software (see Tables 2 and 3 for instrument-specific settings). 1. FOR HTRF CERTIFIED READER. Lysates are then transferred to the assay plate for the detection of total-NFkB by HTRF. + 4 µL HTRF ® pre-mixed antibodies . BMG LABTECH microplate reader; Early passage Human Osteoblast cells (HOb 406-05a), supplied by the European Collection of Cell Cultures ECACC were seeded onto substrates A and B at a density of 10 4 per sample. It can also be further streamlined toLysates are then transferred ®to the assay plate for the detection of Total TYK2 by HTRF reagents. Depending on cell lines used, it can be necessary to dilute the cell lysate to ensure samples are within the assay linear range FOR HTRF CERTIFIED READER. For more ® information about HTRF compatible readers and for set-up recommendations, please. • Our fastest benchtop plate reader – uses two detectors to enable simultaneous, dual-wavelength reading • Well established for laser-based Alpha and TRF technologies, as well as ultrasensitive luminescence • Time-resolved fluorescence certified for use with HTRF ® technology • Software that can facilitate 21 CFR Part 11 compliance for Cisbio’s 96-well low volume plates can be used with all HTRF assays, for example second messengers, phospho-proteins, cytokines and biomarkers. FOR HTRF CERTIFIED. Lysates are then transferred to ® the assay plate for the detection of Total EGFR L858R by HTRF reagents. Quantify up to 10 analytes per well. 10 µL Standard or Sample. 5 hours before 3 more washing steps. Specialized filters allow for IR fluorescence. HTRF TWO-PLATE ASSAY PROTOCOL FOR SUSPENSION CELLS: ONE-PLATE ASSAY PROTOCOL: overnight * * Depending on cell lines used, volume of lysis should be optimized. For more information about HTRF. If not, our scientific team will help you choose the instrument that best suits your needs and budget. Assay setup for a 384-well low-volume plate. Certified SpectraMax® M5e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays’ HTRF (Homogeneous Time-Resolved Fluorescence) technology. The plate map included a low and a high level calibrator, conjugate blanks, and a specific control to assess the counting rate at 620 nm (i. 9. Certified SpectraMax ® M5 e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays HTRF (Homogeneous Time-Resolved Fluorescence) technology. FOR HTRF CERTIFIED READER For more. • HTRF®-Certified Reader. no. Transcreener® ADP2 FP assay. ONE-PLATE ASSAY PROTOCOL: Small volume detection plate 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies Read & analyse Incubate overnight at RT Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. Depending on cell lines used, it can be necessary to dilute the cell lysate to ensure samples are within the assay linear range FOR HTRF CERTIFIED READERFOR HTRF CERTIFIED READER. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. Lysates are then transferred to the assay plate for the detection of total cRaf by HTRF. * For HTRF microplate recommendations, please visit . Read other about this measurement approach here. 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies. Reader. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. Incubate overnight at RT. Assay plates were incubated at RT and read at different time points thereafter on an HTRF ® certified. Data Analysis HTRF®-certified, DLReady, and SpectraMax® DuoLuc™-certified. This protocol gives the cells viability and confluence to be monitored. Our microplates and accessories are manufactured under strict process controls guaranteeing consistent product performance for. Table 1. Download the brochure to learn how this compact and robust device can handle various detection modes, plate formats, and software options. HTRF is a versatile TR-FRET technology developed by Cisbio for detecting biomolecular interactions. The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. ** The Detection Buffer is used to prepare working solutions of acceptor and donor reagents. If not, our scientific team will help you choose the instrument that best suits your needs and budget. These plates can be read on all HTRF certified readers, thus making their implementation in. Suggested plate map: 1: 2 3: A: 14 μL Std 0: 3 μL IP1 d2 Reagent 3 μL IP1 Tb Cryptate AntibodyCisbio’s 96-well low volume plates can be used with all HTRF assays, for example second messengers, phospho-proteins, cytokines and biomarkers. Make sure the setup for Tb Cryptate is used. FOR HTRF CERTIFIED. EnVision Nexus ultimode Plate Reader 2 Benefits • Provides the speed and sensitivity to boost your lab’s productivity with ultra-high throughput • Freely configurable and upgradable to adapt to your needs today and tomorrow • Fully automatable for walkaway convenience • Certified for use with HTRF® technology Key featuresBMG LABTECH plate readers certified for LanthaScreen detection include the PHERAstar FSX, CLARIOstar Plus and VANTAstar. White 384-well low volume plate Make sure to use the set-up for Eu 3+ Cryptate. 1, introducing a time delay of approximately 50 to 150 µ seconds between the system excitation and fluorescence measurement allows the signal to be cleared of all non. The optional HTRF-certified filter-based module may be. Remove the plate sealer and read on an HTRF® compatible reader . best fluorescence polarization plate reader on the market. 1 – 1. A typical HTRF assay uses a europium cryptate as the donor, and the organic fluorophore d2 as the acceptor. Read & analyse. The two-plate protocol involves culturing cells in a 96-well plate before lysis, then transferring lysates into a 384-well low volume detection plate before the addition of Total Cereblon HTRF detection reagents. There are multiple factors to consider including: detection type, additional. 2400 for 665 and 620 with white plate HTRF® Europium cryptate donor / Red acceptor readout. Configure your microplate reader. 1, introducing a time delay of approximately 50 to 150 µ seconds between the system excitation and fluorescence measurement allows the signal to be cleared of all non. The VICTOR® Nivo™ system packs all popular detection modes into the industry’s smallest multimode reader footprint. 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. It can also be further streamlinedFOR HTRF CERTIFIED READER. It utilises an ultra-fast UV/vis spectrometer or filters for absorbance as well as highly sensitive filters for all other detection modes. VICTOR® Nivo™ multimode plate reader 2 | For research use only. Step 1 Step 2 Step 3 Step 4 Step 5 Step 6. Depending on cell lines used, it can be necessary to dilute the cell lysate to ensure samples are within the assay linear range FOR HTRF CERTIFIED READERFOR HTRF CERTIFIED READER. FOR HTRF CERTIFIED READER For. Microplate readers are only certified if they. The VICTOR Nivo is a HTRF certified microplate reader. An example of a LanthaScreen kinase assay run on a BMG LABTECH plate reader can be seen in the application note " LanthaScreen TR-FRET tyrosine kinase and protein kinase C assay ". Read & analyse. The Varioskan LUX multimode reader has received HTRF™ certification from Cisbio Bioassays and DLReady™ validation from Promega Corporation. Plate 25 μL of cells in 96 half-well plate in your appropriate medium. Incubate 4h at RT. protocol with suspension cells or with a. Dual monochromators for assay flexibility With SpectraMax Multi-Mode Readers, there is no need to utilize expensive filters to optimize detection levels and background. Figure 3. For more information about set-up and compatible HTRF ® readers, please visit our website at: ® pre-mixed antibodies TWO- PLATE ASSAY PROTOCOL FOR SUSPENSION CELLS: 25 µL cells . The LVis Plate is transparent in the UV-range of light and is compatible with all BMG LABTECH microplate readers. These plates can be read on all HTRF certified readers, thus making their implementation in your experiments really easy. Flexibility combined with the best sensitivity of its class, and our newly developed Enhanced Dynamic Range technology, make it the ideal reader for assay development. -CERTIFIED READER IS NEEDED. Seal the plate and. This protocol enables the cells' viability and confluence to be monitored. For a list of HTRF-compatible readers and set-up recommendations, please visit our website • Small volume (SV) detection microplates - Use white plate only. FOR HTRF CERTIFIED READER. 47 seconds for a 1536-well plate. For more information about HTRF ®The LUMIstar® Omega is BMG LABTECH's powerful dedicated luminescence microplate reader, providing highly sensitive flash and glow assay readings. HTRF is compatible with a large range of available microplate readers. Both readers demonstrated their ability to detect IP1 concentrations according to Cisbio’s acceptance criteria. a one- plate assay protocol. • HTRF®-Certified Reader **. Lysates are then transferred to the assay plate for the detection of total cRaf by HTRF. Microplate reader assays requiring infrared (IR) or red-shifted assays can be read with great sensitivity using the iD5 reader’s filter system. Plate 1 plate HTRF 96-well low volume plate *To prepare working standard solutions, culture medium can be an alternative the diluent. Cell seeding densities of 2,000,000 cells/well are generally sufficient for most cell lines, but. ONE- PLATE ASSAY PROTOCOL: 5 µL cells + 5 µL compounds (or stimulation buffer) + 5 µL agonist + 5 µL HTRF ® pre-mixed antibodies * Depending on cell lines used, volume of lysis should be optimized. HTRF is a versatile TR-FRET technology developed by Cisbio for detecting biomolecular interactions. 96-well culture plateThe assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same . VICTOR® Nivo™ multimode plate reader is the smallest plate reader on the. 4 mg/ml. Incubate overnight at RT. The measurement of HTRF assays is predominantly performed on microplate readers. FOR HTRF CERTIFIED READER For more ® information about HTRF compatible readers and for set-up recommendations, please visit our. HTRF certified . Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. Step 1 Step 2 Step 3 Step 4 Step 5. Cover the plate with a plate sealer. This protocol, HTS designed, allows. Incubate 24 hours at RT. FOR HTRF CERTIFIED READER . The PHERAstar ® FSX is our most sensitive multi-mode reader for high-throughput screening, combining this sensitivity with fast read times. Alpha Technology: The VICTOR Nivo system. Step 1 Step 2 Step 3 Step 4 Step 5 Step 6. Customer Transformation Hub. FOR HTRF CERTIFIED READER For. It also integrates with the powerful SoftMax Pro Software for. 5 nm filter (PerkinElmer 2100-5110) were used. 96-well culture plate 384SV detection plate- Add . To perform the assay, use white plate only. • HTRF®-Certified Reader **. The 2 plate protocol involves culturing cells in a 96-well plate before lysis then transferring lysates to a 384-well low volume detection plate before adding Phospho-ERK (Thr202/Tyr204) HTRF detection reagents. NanoQuant Plate™ allows rapid, straightforward measurement of 16 x 2 µl samples simultaneously without calibration. Homogeneous Time-Resolved Fluorescence (HTRF) . The perfect microplate reader for everyday biochemical and cell-based assays, it measures at any wavelength, with top and bottom reading of all technologies. EnVision Nexus ultimode Plate Reader 2 Benefits • Provides the speed and sensitivity to boost your lab’s productivity with ultra-high throughput • Freely configurable and upgradable to adapt to your needs today and tomorrow • Fully automatable for walkaway convenience • Certified for use with HTRF® technology Key features BMG LABTECH plate readers certified for LanthaScreen detection include the PHERAstar FSX, CLARIOstar Plus and VANTAstar. As shown in Figure 1, the interaction between Tag1-SOS1 and Tag2-KRAS-WT is detected by using anti-Tag1-Terbium (HTRF donor) and anti-Tag2-XL665 (HTRF acceptor). Cisbio’s 96-well low volume plates can be used with all HTRF assays, for example second messengers, phospho-proteins, cytokines and biomarkers. For fluorescent assays, black plates absorb light and reduce background and crosstalk. These plates can be read on all HTRF certified readers, thus making their implementation in your experiments really easy Provider Format Part Numbers Recommended volume Type; Cisbio: 96-w low volume: 6PL96005 – 66PL96025 – 66PL96100: 20-30 µL:The PHERAstar FS is an advanced HTS plate reader designed for all HTRF assays and features the following: All microplate formats up to 3456-well. Plate 25 μL of cells in 96 half-well plate in your appropriate medium. Lysis buffer 1X for assay protocol on tissues, two-plate assay protocol on adherent cells, and standard dilution. The kit includes everything you need: reagents, buffers and a microplate. HTRF®-certified, DLReady, and SpectraMax® DuoLuc™-certified. CulturPlate-96: White Opaque 96-well Microplate, Sterile and Tissue Culture Treated. Depending on the cell lines used, it may be necessary to dilute the cell lysate to ensure samples are within the assay linear range. PerkinElmer EnVision Multimode Plate Reader. February 23, 2010, WINOOSKI VT, USA - The Synergy TM H4 Hybrid Microplate Reader from BioTek Instruments is now HTRF® certified. It is typically used to determine the concentration of nucleic acids and proteins. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. The HTRF ratio obtained represents the total binding. DATA REDUCTION & INTERPRETATION 1. For a list of HTRF-compatible readers and set-up recommendations, please visit our website • Small volume (SV) detection microplates - Use white plate only. The Infinite 200 PRO is an easy-to-use multimode plate reader family that offers affordable, high performance detection solutions, referenced in more than 1800 peer reviewed. The perfect microplate reader for everyday biochemical and cell-based assays, it measures at any wavelength, with top and bottom reading of all technologies. HTRF is a proprietary time-resolved fluorescence technology that overcomesThe company offers different HTRF-certified, multidetection microplate readers such as the FLUOstar Omega, POLARstar Omega, CLARIOstar and PHERAstar. It enables you to gain insights you couldn’t achieve with detection measurements alone. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. Customer Transformation Hub. 1) . S. HTRF 96-well low volume plate *To prepare working standard solutions, culture medium can be an alternative the diluent. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. We would like to show you a description here but the site won’t allow us. Add. For more information. It is used as a positive control for Gi applications ( biological models validation,FOR HTRF CERTIFIED READER. HTRF® 96-well low volume plate Ref# 66PL96001 * HTRF® 384-well low volume plate Ref 66PL384025 * Non-binding 96-well black plate HTRF®-Certified Reader **. • Our fastest benchtop plate reader – uses two detectors to enable simultaneous, dual-wavelength reading • Well established for laser-based Alpha and TRF technologies, as well as ultrasensitive luminescence • Time-resolved fluorescence certified for use with HTRF ® technology • Software that can facilitate 21 CFR Part 11 compliance for Choose from over 50 certified or compatible plate readers More than 50 certified or compatible instruments, and counting! Your lab might even already have one. Each well requires 10 μL of 4X lysis buffer /well. VICTOR Nivo Multimode Plate Reader • Compact and lightweight • High performance for routine applications, including biochemical and cell-based assays • All major detection. HTRF combines time-resolved fresh detection with FRET and is mainly used for drug screening in high-throughput. For more information about HTRF. The signals are produced by, converted by, or transmitted through the. 96-well or 384-well small volume (SV) detection. The plate is set up for reading and images are acquired according to specified parameters. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. Microplate reader assays requiring infrared (IR) or red-shifted assays can be read with great sensitivity using the iD5 reader’s filter system. As shown in Figure 1, the interaction between Tag1-SOS1 and Tag2-KRAS-WT is detected by using anti-Tag1-Terbium (HTRF donor) and anti-Tag2-XL665 (HTRF acceptor). Plate 25 μL of cells in. e. The EnVision XCite multimode plate reader provides exceptional sensitivity across all detection technologies, while keeping you flexible with monochromator option and full upgradeability. ONE-PLATE ASSAY PROTOCOL: Small volume detection plate. TWO-PLATE ASSAY PROTOCOL FOR SUSPENSION CELLS: + 10 µL lysis buffer ONE-PLATE ASSAY PROTOCOL: Add *Depending on the cell lines used, the lysis volume should be optimized. Microplate reader assays requiring infrared (IR) or red-shifted assays can be read with great sensitivity using the iD5 reader’s filter system. FOR HTRF CERTIFIED READER. FOR HTRF CERTIFIED READER For more ® information about HTRF compatible readers and for set-up recommendations, please visit our. HTRF is a proprietary time-resolved fluoresce nce technology that overcomes To prevent evaporation during assay incubation steps, we recommend the use of TopSeal-A PLUS, a clear adhesive seal for microplate. The dual measurement. For more information about microplate recommendations, please visit our website STORAGE AND STABILITYDetection. HTRF® Europium cryptate donor / Red acceptor readout Setup recommendations for Tristar 5FOR HTRF CERTIFIED READER. HTRF is a proprietary time-resolved fluorescence technology that overcomes Results summary for HTRF IP1 standard curve. Read & analyse. It guarantees high level of cAMP accumulation in the cells. • HTRF plate validated (QC with reference HTRF assays) • Low to high volumes (10 µL to 350 µL) • Can be used with all HTRF compatible readersFOR HTRF CERTIFIED READER. Assay detection requires a microplate reader with. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. Lysates are then transferred to the assay plate for the detection of total-EGFR by HTRF. Its. HTRF plate reader. It combines standard FRET technology with time-resolved measurement of fluorescence, eliminating short-lived background. Read & analyse. Lysates are then transferred to the assay plate for the detection of total-Tau by HTRF. Already more than 50 certified or compatible instruments, and counting! HTRF is compatible with a large range of available microplate readers and certainly the detection system already in your lab. The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. Make sure the setup for Tb Cryptate is used. SpectraMax Paradigm Microplate Reader is a versatile and customizable device that can perform a wide range of detection modes, such as absorbance, fluorescence, luminescence, time-resolved fluorescence, and AlphaScreen. If not, our scientific team will help you choose the instrument that best suits your needs and budget. The EnVision XCite Plate Reader provides exceptional sensitivity and speed with minimal downtime - backed by our global network of application scientists and engineers. With this certification, Cisbio Bioassays guarantees that the Synergy H4 meets or exceeds strict performance and functional criteria for optimal HTRF® readout. 96-well culture plateFOR HTRF CERTIFIED READER For more information about HTRF. FOR HTRF CERTIFIED READER. • HTRF cAMP HiRange Assay Kit (Cisbio Bioassays, France) • 96-well half area plates (white) (Greiner Bio-One, Germany) • 384-well small volume plates (white and black) (Greiner Bio-One, Germany) HTRF RCK The HTRF RCK is used to confirm the general suitability of a reader for HTRF assays, as well as set up and performance validation. It measures 96-well plates in less than 20 seconds and 1536-well formats in under one minute. Seal the plate and incubate 3 hours at RT . The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. HTRF is a proprietary time-resolved fluoresce nce technology that overcomes• HTRF cAMP HiRange Assay Kit (Cisbio Bioassays, France) • 96-well half area plates (white) (Greiner Bio-One, Germany) • 384-well small volume plates (white and black) (Greiner Bio-One, Germany) HTRF RCK The HTRF RCK is used to confirm the general suitability of a reader for HTRF assays, as well as set up and performance validation. The PHERAstar FS is an advanced HTS plate reader designed for all HTRF assays and features the following: All microplate formats up to 3456-well. For more information about HTRF. You can verify your reader’s ability to detect and use HTRF technology, then easily configure your instrument with its own HTRF setup. FOR HTRF CERTIFIED READER For more information about HTRF. 5 EXAMPLE OF MAP PLATE . For more information. PerkinElmer’s 96w low volume, 96w and 384w microplates can be read on all HTRF certified readers, thus making their implementation in your experiments really easy. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same . • HTRF®-Certified Reader **. High-end TR-FRET/HTRF® performance: certified ®to measure HTRF assays in both black or white plates, the reader guarantees no compromises in any HTRF assay. If not, our scientific team will help you choose the instrument that best suits your needs and budget. LysisAdd ONE-PLATE ASSAY PROTOCOL: Small volume detection plate. It can also be further streamlined toFOR HTRF CERTIFIED READER. • User-upgradable microplate reader in under 2 minutes • Array of detection cartridge applications at your fingertips. Lysates are then transferred to the assay plate for the detection of total-RET by HTRF. 96-well culture plateFor each fluorescent ligand concentration, the nonspecific binding was determined by adding a large excess of unlabeled spiperone. HTRF TWO-PLATE ASSAY PROTOCOL FOR SUSPENSION CELLS: Add ONE-PLATE ASSAY PROTOCOL: overnight * * Depending on cell lines used, volume of lysis should be optimized. Catalog No. 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies.